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Histology & Biomarker Core

Overview of Services

The Histology & Biomarker Core provides state-of-the-art tissue biomarker histologic detection and image analyses for both human and mouse tissue biospecimens.


HISTOLOGY

Histology services include tissue processing, embedding, sectioning, H&E staining, select special stains, TMA construction, training, and consultation.

BIOMARKER STAINING

Our laboratory offers a robust menu of immunohistochemistry (IHC) and immunofluorescence (IF) single and multiplex panel stains on the Ventana Discovery Ultra platform.

  • We also offer the optimization of new single stains in DAB or other chromogens and multiplex panels
  • Antibodies generated in rabbit, mouse, and rat hosts can be used.
  • Our stripping protocols allow for panel construction with multiple antibodies generated in the same host species.
  • Multiplex IHC chromogenic panels can be built with up to three markers. The tryramide based translucent dyes used offer a distinct color shift with marker co-localization.
  • IF panels can be constructed with up to 5 markers using Ventana’s staining technology or 6 markers using Akoya’s OPAL dyes.
  • Current list of protocols can be found here: Biomarker Menu

WHOLE SLIDE SCANNING

Digital imaging services include microscopic whole slide scanning of both brightfield (BF) and immunofluorescent (FL) slides as well multispectral region of interest (ROI) scanning.

  • Zeiss Axio ScanZ.1 - Whole Slide Scanning Service
    • Automated loading of up to 100 slides
    • Fluar5x and Plan-Apochromat 10x/20x/40x objective scanning for BF and FL 
    • 9 fluorescent channels: Cy7, Cy5, Red 610, Alexa 594/Texas Red/RFP, Cy3, FITC/GFP, DCC/Coumarin, Rhodamine 6G, DAPI
  • Akoya Vectra 3.0 - Rental
    • Automated loading of up to 6 slides
    • 5x, 10x, 20x, 40x objective scanning BF or FL (multi-spectral/color)
    • 5 filter cubes: FITC/AF488, Cy3/TITC/AF555, DAPI, AF594/Texas Red, Cy5/-ET

For more information please see our Imaging/Scanning FAQs

 

Leadership

Jennifer Bolen | Core Manager | 415-476-0435 (office) | 415-514-3500 (lab) | jennifer.bolen@ucsf.edu

Links and Resources

  1. Cancer Center Core Website
  2. Biospecimen Resource Program (BIOS)
  3. UCSF Core Services and Equipment Search

Location and available hours

Location Open Lab Hours

UCSF Mt. Zion Campus

2340 Sutter Street, Room S-241

San Francisco, CA 94115

 

  Monday through Friday

  9:00AM to 3:30PM

Contacts

Name Role Phone Email Location
Histology and Biomarker Core
General Inquires
 
415.514.3500
 
hbcore@ucsf.edu
 
2340 Sutter Street, Room S-241
 
Jennifer Bolen
Core Manager
 
415.476.0435
 
Jennifer.Bolen@ucsf.edu
 
2340 Sutter Street, Room S-241
 
Mohammad Naser
Image Analysis & Imaging (Akoya Vectra Scanner)
 
415.514.3500
 
Mohammad.Naser@ucsf.edu
 
2340 Sutter Street, Room S-241
 
Kristine Wong
Biomarkers & Imaging
 
415.514.3500
 
Kristine.Wong@ucsf.edu
 
2340 Sutter Street, Room S-241
 
Rosa Navarro
Cancer Center Cores Administrative Support
 
415.502.3723
 
Rosa.Ruiz@ucsf.edu
 

 
Scott Myers
Histology & Tissue Microarray
 
415.514.3500
 
scott.myers2@ucsf.edu
 
2340 Sutter Street, Room S-241
 

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