New Rates Effective 07-01-2022

Overview of Services

The Histology & Biomarker Core provides state-of-the-art tissue biomarker histologic detection and imaging for both human and mouse tissue biospecimens.

 

BIOMARKER STAINING

Our laboratory offers a robust menu of immunohistochemistry (IHC) and immunofluorescence (IF) single and multiplex panel stains on the Ventana Discovery Ultra platform.

• We also offer the optimization of new single stains in DAB or other chromogens and multiplex panels
• Antibodies generated in rabbit, mouse, and rat hosts can be used.
• Our stripping protocols allow for panel construction with multiple antibodies generated in the same host species.
• Multiplex IHC chromogenic panels can be built with up to three markers. The tryramide based translucent dyes used offer a distinct color shift with marker co-localization.
• IF panels can be constructed with up to 5 markers using Ventana’s staining technology or 6 markers using Akoya’s OPAL dyes.
• Current list of protocols can be found here: Biomarker Menu

WHOLE SLIDE SCANNING

Digital imaging services include microscopic whole slide scanning of both brightfield (BF) and immunofluorescent (FL) slides as well multispectral region of interest (ROI) scanning.

• Zeiss Axio ScanZ.1 - Whole Slide Scanning Service
• Automated loading of up to 100 slides
• Fluar5x and Plan-Apochromat 10x/20x/40x objective scanning for BF and FL 
• 9 fluorescent channels: Cy7, Cy5, Red 610, Alexa 594/Texas Red/RFP, Cy3, FITC/GFP, DCC/Coumarin, Rhodamine 6G, DAPI
• Akoya Vectra 3.0 - Rental
• Automated loading of up to 6 slides
• 5x, 10x, 20x, 40x objective scanning BF or FL (multi-spectral/color)
• 5 filter cubes: FITC/AF488, Cy3/TITC/AF555, DAPI, AF594/Texas Red, Cy5/-ET

For more information please see our Imaging/Scanning FAQs

 

Leadership

Dr. Scott Kogan | Core Director | scott.kogan@ucsf.edu

Links and Resources

1. Cancer Center Core Website
2. Biospecimen Resource Program
3. UCSF Core Services and Equipment Search

Location and available hours

Location	Open Lab Hours
UCSF Mt. Zion Campus 2340 Sutter Street, Room S-241 San Francisco, CA 94115	Monday through Friday   9:00AM to 3:30PM Excluding UCSF Holidays

Contacts

Name	Role	Phone	Email	Location
Histology and Biomarker Core	General Inquires	415.514.3500	hbcore@ucsf.edu	2340 Sutter Street, Room S-241
Kristine Wong	Biomarkers & Imaging	415.514.3500	Kristine.Wong@ucsf.edu	2340 Sutter Street, Room S-241
Rosa Navarro	Cancer Center Cores Administrative Support	415.502.3723	Rosa.Ruiz@ucsf.edu
Dr. Scott Kogan	Core Director		scott.kogan@ucsf.edu
Dr. Mohammad Naser	Analysis & Biomarkers		mohammad.naser@ucsf.edu

Map